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pei max transfection reagent  (Kyfora Bio)


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    Structured Review

    Kyfora Bio pei max transfection reagent
    Pei Max Transfection Reagent, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 5715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyethylenimine/PEI+MAX+Transfection+Reagent/custom%4024765-1%4010%2E1016%2Fj%2Emtbio%2E2026%2E103524
    Average 99 stars, based on 5715 article reviews
    pei max transfection reagent - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: SIRT7 links H3K36ac epigenetic regulation with genome maintenance in the mouse testis.
    Article Snippet: WT and Sirt7-/- GC-2spd(ts) (ATCC CRL-2196) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, 31966-021) supplemented with 10% FBS (Gibco, 10270-106) and 1% PenStrep (BioWest, l0022-100) at 37oC, in 5% CO2.

    Article Title: Targeting cancer-associated cell surface RNAs with oligonucleotide-drug conjugates enables broad antitumor activity
    Article Snippet: Polyethylenimine (Polysciences, 23966-100).

    Transfection:

    Article Title: Systematic design of combination therapy by targeting master regulators of coexisting diffuse midline glioma cell states.
    Article Snippet: Lentivirus was produced in HEK293T cells maintained in DMEM (Gibco, 11995073) with 10% FBS (Sigma-Aldrich, F2442). .. Cells were transfected using 18 μl of 2 mg ml−1 polyethylenimine (PEI 25000; Polysciences, 23966-1) with 6 μg plasmid DNA, 4.5 μg psPAX2 (Addgene, 12260) and 1.5 μg pMD2.G (Addgene, 12259) mixed in Opti-MEM (Gibco, 31985062) per 10 cm dish. .. Media was replaced 12 h post transfection, and viral supernatant was collected at 48 h and 72 h, filtered (0.45 μm), precipitated with PEG-6000 (Santa Cruz Biotechnology, sc-302016), resuspended and pooled.

    Article Title: SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia to facilitate micro-size platelet clotting
    Article Snippet: .. Plasmid encoding for Hexaprolin spike FL trimer was transiently transfected into HEK293-T cells at a final concentration of 3 μg/mL in Freestyle 293 Expression Medium using polyethylenimine (PEI, Polysciences) as a transfection reagent. .. After 24 h, the cells were diluted 1:1 with ExCell 293 Serum-Free Medium for HEK 293 cells supplemented with 2.2 mM valproic acid to promote additional endocytic uptake of the plasmid-PEI complex.

    Article Title: Kdm4dl is expressed in adult mouse testis and encodes a functional KDM4D-like protein.
    Article Snippet: HEK293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Co.; 041-30081) supplemented with 10% fetal bovine serum (FBS) (BioWest; S1810) and penicillin–streptomycin (Nacalai Tesque; 09367-34) at 37 ◦C in a humidified atmosphere containing 5% CO2. .. Cells were transiently transfected with equimolar amounts of expression plasmids encoding Kdm4dl-HA, Kdm4d-HA, or GFP using polyethylenimine (PEI; Polysciences, 24765) in 6-well plates. ..

    Article Title: CELLISA – a cell-cell binding assay for evaluation of nanovesicle targeting proteins
    Article Snippet: .. For most sCELLISAs, cells were transfected using polyethylenimine (PEI Max from Polysciences #24765, dissolved at 1 mg/mL in DI H 2 O, pH adjusted to 7.0 with hydrochloric acid, and 0.22 μm-filtered) at a 4:1 by mass PEI Max to DNA ratio. .. A BZ-x800 microscope running BZ Series Application (v01.01.00.17, Keyence) with a PlanApo4x (NA 0.2) objective was used.

    Plasmid Preparation:

    Article Title: Systematic design of combination therapy by targeting master regulators of coexisting diffuse midline glioma cell states.
    Article Snippet: Lentivirus was produced in HEK293T cells maintained in DMEM (Gibco, 11995073) with 10% FBS (Sigma-Aldrich, F2442). .. Cells were transfected using 18 μl of 2 mg ml−1 polyethylenimine (PEI 25000; Polysciences, 23966-1) with 6 μg plasmid DNA, 4.5 μg psPAX2 (Addgene, 12260) and 1.5 μg pMD2.G (Addgene, 12259) mixed in Opti-MEM (Gibco, 31985062) per 10 cm dish. .. Media was replaced 12 h post transfection, and viral supernatant was collected at 48 h and 72 h, filtered (0.45 μm), precipitated with PEG-6000 (Santa Cruz Biotechnology, sc-302016), resuspended and pooled.

    Article Title: SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia to facilitate micro-size platelet clotting
    Article Snippet: .. Plasmid encoding for Hexaprolin spike FL trimer was transiently transfected into HEK293-T cells at a final concentration of 3 μg/mL in Freestyle 293 Expression Medium using polyethylenimine (PEI, Polysciences) as a transfection reagent. .. After 24 h, the cells were diluted 1:1 with ExCell 293 Serum-Free Medium for HEK 293 cells supplemented with 2.2 mM valproic acid to promote additional endocytic uptake of the plasmid-PEI complex.

    Article Title: Dual E3 ligase recruitment by monovalent degraders for tunable SMARCA 2/4 degradation.
    Article Snippet: .. Semiconfluent Lenti-X cells in 10-cm dishes were cotransfected with 1 μg of the envelope plasmid pMD2.G (Addgene, 12259), 2 μg of packaging plasmid psPAX2 (Addgene, 12260) and 4 μg of the lentiviral plasmid using polyethylenimine (PEI MAX, molecular weight: 40,000; Polysciences). ..

    Concentration Assay:

    Article Title: SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia to facilitate micro-size platelet clotting
    Article Snippet: .. Plasmid encoding for Hexaprolin spike FL trimer was transiently transfected into HEK293-T cells at a final concentration of 3 μg/mL in Freestyle 293 Expression Medium using polyethylenimine (PEI, Polysciences) as a transfection reagent. .. After 24 h, the cells were diluted 1:1 with ExCell 293 Serum-Free Medium for HEK 293 cells supplemented with 2.2 mM valproic acid to promote additional endocytic uptake of the plasmid-PEI complex.

    Expressing:

    Article Title: SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia to facilitate micro-size platelet clotting
    Article Snippet: .. Plasmid encoding for Hexaprolin spike FL trimer was transiently transfected into HEK293-T cells at a final concentration of 3 μg/mL in Freestyle 293 Expression Medium using polyethylenimine (PEI, Polysciences) as a transfection reagent. .. After 24 h, the cells were diluted 1:1 with ExCell 293 Serum-Free Medium for HEK 293 cells supplemented with 2.2 mM valproic acid to promote additional endocytic uptake of the plasmid-PEI complex.

    Article Title: Kdm4dl is expressed in adult mouse testis and encodes a functional KDM4D-like protein.
    Article Snippet: HEK293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Co.; 041-30081) supplemented with 10% fetal bovine serum (FBS) (BioWest; S1810) and penicillin–streptomycin (Nacalai Tesque; 09367-34) at 37 ◦C in a humidified atmosphere containing 5% CO2. .. Cells were transiently transfected with equimolar amounts of expression plasmids encoding Kdm4dl-HA, Kdm4d-HA, or GFP using polyethylenimine (PEI; Polysciences, 24765) in 6-well plates. ..

    Molecular Weight:

    Article Title: Dual E3 ligase recruitment by monovalent degraders for tunable SMARCA 2/4 degradation.
    Article Snippet: .. Semiconfluent Lenti-X cells in 10-cm dishes were cotransfected with 1 μg of the envelope plasmid pMD2.G (Addgene, 12259), 2 μg of packaging plasmid psPAX2 (Addgene, 12260) and 4 μg of the lentiviral plasmid using polyethylenimine (PEI MAX, molecular weight: 40,000; Polysciences). ..



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